CD8A Antibody / CD8 alpha

• Description:

  CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha) . A majority of thymocytes and a subpopulation of mature T cells and NK cell express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P01732

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment from the C-terminal cytoplasmic domain of the alpha chain of human CD8 was used as the immunogen for the CD8A antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  IHC-P

• Purity:

  Protein A affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD8A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD8A antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD8A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD8A antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD8A antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.