Cd8 alpha Antibody

CAT: 0800-RQ4104Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-RQ4104Size:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
CAS Number
9007-83-4
Specifications
Western Blot: 0.5-1 µg/mL, IHC (FFPE) : 1-2 µg/mL, FACS: 1-3ug/10^6 cells, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P01731
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
A recombinant mouse partial protein corresponding to amino acids K28-Y196 was used as the immunogen for the Cd8 alpha antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, FACS, ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the Cd8 alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This Cd8 alpha antibody is available for research use only.
Storage Conditions
After reconstitution, the Cd8 alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Cd8 alpha antibody should be determined by the researcher.
Location
Cytoplasmic, membrane, secreted
Image Legend
Western blot testing of 1) rat spleen and 2) rat thymus with Cd8a antibody at 0.5ug/ml. Expected molecular weight: 26-40 kDa depending on glycosylation level.

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