Cdk7 Antibody

• Description:

  Cyclin-dependent kinase 7, also known as Cell division protein kinase 7, is an enzyme that in humans is encoded by the CDK7 gene. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. The gene was assigned to human chromosome 5q13.2. Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. It is required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  P50613

• Host:

  Rabbit

• Reactivity:

  Human, Rat, Mouse

• Immunogen:

  An amino acid sequence from the C-terminus of human Cyclin-dependent kinase 7 (RKRTEALEQGGLPKKLIF) was used as the immunogen for this Cdk7 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the Cdk7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Cdk7 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Cdk7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Cdk7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Cdk7 antibody and Lane 1: HeLa; 2: MCF-7; 3: A549; 4: COLO320; 5: Jurkat cell lysate. Expected/observed molecular weight ~37 kDa.