CDK2 Antibody

• Description:

  In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclindependent kinase 2 (Cdk2), also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2-related cyclin dependent kinases have been identified. These include Cdk3, Cdk4, Cdk5, PCTAIRE-1, PCTAIRE-2, PCTAIRE-3, Cdk6 Cdk7, Cdk8 and KKIALRE.

• Specifications:

  Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/10^6 cells in 0.1ml

• UniProt:

  P24941

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant human CDK2 was used as the immunogen for this CDK2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  AN4.3

• Applications:

  WB, FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CDK2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CDK2 antibody is available for research use only.

• Storage Conditions:

  Store the CDK2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CDK2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CDK2 antibody (clone AN4.3) as confirmation of integrity and purity.