CDK2 Antibody

• Description :

  CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs) . Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity) . [UniProt]

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50

• UniProt :

  P24941

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 230-260 from the human protein was used as the immunogen for this CDK2 antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, FACS, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution :

  Aliquot the CDK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This CDK2 antibody is available for research use only.

• Storage Conditions :

  Aliquot the CDK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Mouse, Rat, Bovine, Hamster, Xenopus

• CAS Number :

  9007-83-4

• Image Legend :

  IHC analysis of FFPE human lung carcinoma stained with CDK2 antibody