CDK1 Antibody

• Description:

  CDC2, Cell Division Cycle 2, is also known as CDK1 (Cyclin-dependent Kinase 1) . CDC2 is a catalytic subunit of a protein kinase complex, called the M-phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In HeLa cells CDC2 is the most abundant phosphotyrosine-containing protein and its phosphotyrosine content is subject to cell cycle regulation. CDC2 gene is located on chromosome 10.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Flow cytometry: 1-3ug/million cells

• UniProt:

  P06493

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A human recombinant protein (amino acids L66-M297) was used as the immunogen for the CDK1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  2G11

• Applications:

  WB, IHC-P, FACS

• Purity:

  Affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide

• Reconstitution:

  After reconstitution, the CDK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This CDK1 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the CDK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the CDK1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  Flow cytometry testing of human U-2 OS cells with CDK1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= CDK1 antibody.