CDK5 Antibody

• Description:

  Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity.[UniProt]

• Specifications:

  Flow Cytometry: 1:25, Immunofluorescence: 1:25, Western blot: 1:1000

• UniProt:

  Q00535

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This CDK5 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 254-289 amino acids from the C-terminal region of human CDK5.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  FACS, IF, WB, ELISA

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the CDK5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CDK5 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CDK5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the CDK5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Rat, Bovine, Xenopus

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from A431, HeLa, K562, mouse NIH3T3 cell line using CDK5 antibody