ABCG4 Antibody

• Description :

  ATP-Binding Cassette, Subfamily G, Member 4 is a protein that in humans is encoded by the ABCG4 gene. The protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. This protein is a member of the White subfamily and is expressed predominantly in liver tissue. By genomic sequence analysis, Engel et al. (2001) mapped the gene to chromosome 11q23.3. Engel et al. (2001) demonstrated 5-fold induction of ABCG4 following treatment of normal monocyte-derived macrophages with the LXR and RXR agonists 9-cis retinoic acid and 22R hydroxycholesterol. Removal of cholesterol from macrophages by cyclodextrin decreased ABCG4 message levels.

• Specifications :

  Western blot: 0.5-1 µg/mL

• UniProt :

  Q9H172

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  An amino acid sequence from the middle region of human ABCG4 (AVQNGLCAMAEKKSS) was used as the immunogen for this ABCG4 antibody.

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  WB

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the ABCG4 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This ABCG4 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the ABCG4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the ABCG4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot testing of ABCG4 antibody; Lane 1: rat brain; 2: mouse brain tissue lysate. Predicted molecular weight 60-80 kDa