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MKK7 Antibody / MAP2K7 / MEK7

CAT: 0800-R31022Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31022Size:100 µg
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Description
MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7 (MAPKK7), JNKK2, PRKMK7, Mek7 and MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms) . Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53) . MKK7 / Mek7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. the protein was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 / Mek7 exerts its tumor suppressive function through p53.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O14733
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Immunogen
An amino acid sequence from the N-terminus of human MEK7/MKK7 (ARRRIDLNLDISPQRPR) was used as the immunogen for this MKK7 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Reconstitution
After reconstitution, the MKK7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MKK7 antibody is available for research use only.
Storage Conditions
After reconstitution, the MKK7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the MKK7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) human HeLa, 2) monkey COS-7, 3) rat skeletal muscle and 4) mouse skeletal muscle tissue lysate with MKK7 antibody. Predicted molecular weight ~47 kDa.

UniProtKB · O14733

Dual specificity mitogen-activated protein kinase kinase 7

MP2K7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O14733
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MAP2K7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MAP kinase kinase 7, MAPKK 7
EC number
2.7.12.2
Processing
—
Secondary accessions
B2R9S5, D6W659, O14648, O14816, O60452, O60453, Q1PG43, Q8IY10
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisStress response

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Domain

Coiled coil

Cellular component

CytoplasmNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferaseTyrosine-protein kinase

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