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MKK7 Antibody / MEK7 / MAP2K7

CAT: 0800-R31800Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R31800Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. [UniProt]
CAS Number
9007-83-4
Specifications
Western blot: 0.1-0.5 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence (FFPE) : 5 µg/mL, Flow cytometry: 1-3ug/million cells
UniProt
O14733
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids AASSLEQKLSRLEAKLKQENREARRRIDLNLDISPQRPR of human MAP2K7 were used as the immunogen for the MKK7 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, IF, FACS
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Reconstitution
After reconstitution, the MKK7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This MKK7 antibody is available for research use only.
Storage Conditions
After reconstitution, the MKK7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the MKK7 antibody should be determined by the researcher.
Location
Cytoplasmic, nuclear
Image Legend
Immunofluorescent staining of FFPE human PC-3 cells with MKK7 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

UniProtKB · O14733

Dual specificity mitogen-activated protein kinase kinase 7

MP2K7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O14733
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MAP2K7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
MAP kinase kinase 7, MAPKK 7
EC number
2.7.12.2
Processing
—
Secondary accessions
B2R9S5, D6W659, O14648, O14816, O60452, O60453, Q1PG43, Q8IY10
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisStress response

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Domain

Coiled coil

Cellular component

CytoplasmNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferaseTyrosine-protein kinase
Product Datasheet
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