ABCG2 Antibody (BCRP)

ATP-binding cassette, subfamily g, member 2, also known as ABCP, BCRP or MRX, is aprotein that in humans is encoded by the ABCG2gene. Thegene encodes a membrane transporter belonging to the ATP-binding cassette (ABC) superfamily of membrane transporters, which are involved in the trafficking of biologic molecules across cell membranes.Theprotein is also a high capacity transporter for uric acid excretion in the kidney, liver, and gut. The ABCG2 gene is mapped on 4q22.1. In vitro assays of isolated membrane preparations revealed a high-capacity, vanadate-sensitive ATPase activity associated withABCG2expression that was stimulated by compounds known to be transported by this protein. Ozvegy et al. (2001) concluded thatthe proteinis likely functioning as a homodimer or homooligomer in this expression system since it is unlikely that putative Sf9 transport partners would be overexpressed at similarly high levels.Abcg2transports pheophorbide-a, which occurs in various plant-derived foods and food supplements and is highly efficient in limiting its uptake from ingested food.ABCG2is a major factor in the concentrative transfer of drugs, carcinogens, and dietary toxins to the milk of mice, cows, and humans.

9007-83-4

Western blot: 0.5-1 µg/mL

Q9UNQ0

Human

An amino acid sequence from the middle region of human BCRP/ABCG2 (NHEKNERINRVIQEL) was used as the immunogen for this ABCG2 antibody.

WB

Antigen affinity

Antigen affinity purified

Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

After reconstitution, the ABCG2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

This ABCG2 antibody is available for research use only.

After reconstitution, the ABCG2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

The stated application concentrations are suggested starting points. Titration of the ABCG2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.