TRPC3 Antibody

CAT: 0800-R30846Size: 100 µgDry Ice: NoHazardous: No
CAT#:0800-R30846Size:100 µg
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Description
Transient receptor potential cation channel, subfamily C, member 3, also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, the protein behaved as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. It is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
Q13507
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Amino acids 836-851 (HSFNSILNQPTRYQQI) were used as the immunogen for this TRPC3 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the TRPC3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This TRPC3 antibody is available for research use only.
Storage Conditions
After reconstitution, the TRPC3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the TRPC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Location
Membrane
Image Legend
Western blot testing of TRPC3 antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) intestine; 4: (r) ovary; 5: human U87; 6: (h) A549; 7: (h) COLO320; 8: (h) SW620; 9: (h) HeLa lysate. Predicted molecular weight ~96/106/97 kDa (isoforms 1/2/3), observed here at ~120 kDa.

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