Cdk5 Antibody

• Description:

  Cyclin-Dependent Kinase 5, is also know as PSSALRE. The p34 (CDC2) protein kinase regulates important transitions in the eukaryotic cell cycle. Cdk5 expression is in all human tissues and cell lines tested. The the gene is mapped to chromosome 7q36. Cyclin-dependent kinase 5 is predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau. It is required for proper development of the mammalian central nervous system. To be activated, Cdk5 must associate with its regulatory subunit, p35 (CDK5R1) . It can phosphorylate DARPP32 at threonine-75, converting it into an inhibitor of PKA.

• Specifications:

  Western blot: 0.5-1 µg/mL

• UniProt:

  Q00535

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  An amino acid sequence from the C-terminus of human Cdk5 (QRISAEEALQHPYFSDFCPP) was used as the immunogen for this Cdk5 antibody (100% homologous in human, mouse and rat) .

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB

• Purity:

  Antigen affinity

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution:

  After reconstitution, the Cdk5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This Cdk5 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the Cdk5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  The stated application concentrations are suggested starting amounts. Titration of the Cdk5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of Cdk5 antibody and Lane 1: HeLa; 2: Jurkat cell lysate