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ERK2 Antibody / MAPK1

CAT: 0800-R30367Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30367Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene. The gene is mapped to 22q11.2. And The protein encoded by this gene is a member of the MAP kinase family. The deduced 360-amino acid human ERK2 protein shares 98% identity with rat Erk2. The MAP kinase ERK2 is widely involved in eukaryotic signal transduction. Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets. Futhermore, the ERK pathway is necessary for experience-dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex. And the MAPK pathway is also involved in responses to NTN1.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL
UniProt
P28482
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminus of human ERK2 (FEETARFQPGYRS) was used as the immunogen for this ERK2 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the ERK2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ERK2 antibody is available for research use only.
Storage Conditions
After reconstitution, the ERK2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the ERK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of ERK2 antibody and Lane 1: rat brain; 2: rat lung; 3: rat placenta; 4: HT1080; 5: HeLa; 6: MM231; 7: Raji; 8: COLO320 cell lysate. Predicted/observed molecular weight: ~41kDa.

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