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ERK1 Antibody / MAPK3

CAT: 0800-R30409Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30409Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mitogen-activated protein kinase 3 is an enzyme that in humans is encoded by the MAPK3 gene.The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. MAPK3 gene is mapped to human chromosome 16 by hybrid cell panel analysis.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 0.5-1 µg/mL, Immunocytochemistry: 0.5-1 µg/mL
UniProt
P27361
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the middle region of human ERK1 (KTKVAWAKLFPKSDS) was used as the immunogen for this ERK1 antibody (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P, ICC
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the ERK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This ERK1 antibody is available for research use only.
Storage Conditions
After reconstitution, the ERK1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the ERK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of ERK1 antibody and Lane 1: rat spleen; 2: rat thymus; 3: rat skeletal muscle; 4: rat kidney; 5: HeLa; 6: Jurkat; 7: Raji; 8: COLO320. Predicted molecular weight: ~44 kDa.

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