Products for Research Use Only
RegisterLogin

Human ERK2/MAPK1 antibody

CAT: 0112-ATGA0182-100Size: 100 µLDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0112-ATGA0182-100Size:100 µL
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Mitogen-activated protein kinases (MAPKs) are reported to be critical regulatory factors for the growth andmigration of various cell types including vascular smooth muscle cells (VSMCs) . It has also been reported that the activation of the MAP kinase family, extracellular signal regulated kinases 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK) via Src activation is important for AII-induced migration of VSMCs. MAPKs are intracellular signal-transduction pathways that have been shown to play a central role in the development of injury following ischemia in the brain and heart. MAPKs regulate gene expression, which is important in cell injury/repair and proliferation/differentiation.
Product Name Alternative
PRKM2, PRKM1, p42MAPK, p41MAPK, p41, p40, p38, Mitogen-activated protein kinase 1 MAP kinase 2, Mitogen-activated protein kinase 1, MAPK2, MAPK1, Extracellular Signal Regulated Kinase 2, ERT1, ERK2, ERK,
Host
Mouse
Antigen Species
Human
Reactivity
Human
Immunogen
Recombinant human MAPK1 (1-360) purified from E.coli
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
AT1A4
Conjugation
Unconjugated
Applications
ELISA, WB, ICC/IF
Purification Method
By protein-G affinity chromatography
Concentration
1 mg/mL (determined by BCA assay)
Additionnal Information
ERK, ERK 2, ERK2, ERT 1, ERT1, Extracellular Signal Regulated Kinase 2, MAPK 1, MAPK 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 1 MAP kinase 2, p38, p40, p41, p41mapk, P42MAPK, PRKM1, PRKM2, ATGA0182-10 µg, ATGA0182-20 µg, ATGA0182-50 µg, ATGA0182-100 µg, ATGA0182-250 µg, ATGA0182-500 µg, ATGA0182-1 mg, ATGA0182-010, ATGA0182-020, ATGA0182-050, ATGA0182-100, ATGA0182-250, ATGA0182-500, ATGA0182-01M
References & Citations
Le A, et al. (2010) Proc Natl Acad Sci U S A, 107 (5) :2037-42.; ; Gesslein B, et al. (2010) Mol Vis,16:392-407.; ; Lai EW, et al. (2004) J Invest Surg, 17:45-53.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol
Applications Notes
The antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 3000. Recommended starting dilution is 1:500
Scientific Category
Cancer
NCBI Accession Number
NP_620407
Uniprot Accession Number
P28482
WB Description
Cell lysates of HepG2 (35ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK1 (1:3000) . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
IF Description
Immunofluorescence of human MCF7 cells stained with Phalloidin-TRITC (Red) for Actin staining and monoclonal anti-human MAPK1 antibody (1:500) with Alexa 488 (Green) .

Related Products

CatalogName
ATGP0445-020Recombinant human ERK2/MAPK1 protein
ATGP0445-500Recombinant human ERK2/MAPK1 protein
ATGP0445-100Recombinant human ERK2/MAPK1 protein
ATGA0191-100Human ERK1/MAPK3 antibody
ATGA0191-050Human ERK1/MAPK3 antibody
ATGP0687-500Recombinant human ERK1/MAPK3 protein
ATGP0687-020Recombinant human ERK1/MAPK3 protein
ATGP0687-100Recombinant human ERK1/MAPK3 protein
ATGP1649-100Recombinant human JNK2/MAPK9 protein
ATGP3837-020Recombinant human JNK3/MAPK10 protein

Alternative Products

CatalogName
GWB-A8E2A5Extracellular Signal-Regulated Kinase 2 (MAPK1/ERK2) Mouse anti-Human Monoclonal Antibody
32565-05111Human ERK2 Antibody
GWB-C15739Extracellular Signal-Regulated Kinase 2 (MAPK1/ERK2) Mouse anti-Human Monoclonal (4C11C11C4) Antibody
32565-05121Human ERK2 Antibody (Biotin Conjugate)
GTR18188233-01Mouse Monoclonal human ERK2 Antibody
32565-05161Human ERK2 AssayLite™ Antibody (APC Conjugate)
MBS1490658-01Rabbit Anti Human MAPK1 PolyClonal Antibody
32565-05141Human ERK2 AssayLite™ Antibody (FITC Conjugate)
32565-05151Human ERK2 AssayLite™ Antibody (RPE Conjugate)
32565-05171Human ERK2 AssayLite™ Antibody (PerCP Conjugate)

Popular Products