ERK1/2 Antibody / MAPK1/3

• Description:

  Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

• Specifications:

  Western blot: 1:1000

• UniProt:

  P27361, P28482

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthetic peptide corresponding to a portion of human ERK1 was used as the immunogen for this ERK1/2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  LgG1

• Clone:

  4F3-A10-E7

• Applications:

  WB

• Purity:

  Protein G affinity

• Format:

  Purified

• Buffer:

  Antibody in PBS with 50% Glycerol and 0.03% ProClin 300

• Reconstitution:

  Store the ERK1/2 antibody at -20oC.

• Limitations:

  This ERK1/2 antibody is available for research use only.

• Storage Conditions:

  Store the ERK1/2 antibody at -20°C.

• Formulation:

  In PBS with 50% glycerol and 0.03% ProClin 300

• Applications Notes:

  The stated application concentrations are suggested starting points. Titration of the ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot testing of human Jurkat, human A549, rat C6, moouse NIH3T3 and human HeLa cell lysates using ERK1/2 antibody at 1:1000. Predicted molecular weight: ~44 kDa (ERK1), ~42 kDa (ERK2) .