TIMP2 Antibody

• Description :

  TIMP2 gene is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition.

• Specifications :

  IHC (FFPE) : 0.5-1 µg/mL, ELISA: 0.1-0.5 µg/mL (human protein tested) ; request BSA free format for coating

• UniProt :

  P16035

• Host :

  Rabbit

• Reactivity :

  Human, Mouse, Rat

• Immunogen :

  An amino acid sequence from the C-terminus of human TIMP2 (RGAAPPKQEFLDIEDP) was used as the immunogen for this TIMP2 antibody (100% homologous in human, mouse and rat) .

• Clonality :

  Polyclonal

• Isotype :

  IgG

• Applications :

  IHC-P, ELISA (protein)

• Purity :

  Antigen affinity

• Format :

  Antigen affinity purified

• Buffer :

  Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal

• Reconstitution :

  After reconstitution, the TIMP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations :

  This TIMP2 antibody is available for research use only.

• Storage Conditions :

  After reconstitution, the TIMP2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation :

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes :

  The stated application concentrations are suggested starting amounts. Titration of the TIMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, cell surface, secreted

• Image Legend :

  IHC-P: TIMP2 antibody testing of human breast cancer tissue. HIER: steamed with pH6 citrate buffer.