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PKC alpha Antibody

CAT: 0800-R30246Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30246Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein kinase C (PKC) is the major phorbol ester receptor. Activation of PKC by calcium ions and the second messenger diacylglycerol is though to play a central role in the induction of cellular responses to a variety of ligand-receptor systems and in the regulation of cellular responsiveness to external stimuli. Three of these, termed alpha, beta and gamma, are highly homologous. PRKCA1 is mapped to 17q22-q23.2. PKC-alpha regulates cardiac contractility and propensity toward heart failure.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL
UniProt
P17252
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
An amino acid sequence from the C-terminal of human PKC alpha (100% homologous in human, mouse and rat) .
Clonality
Polyclonal
Isotype
IgG
Applications
WB, IHC-P
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal
Reconstitution
After reconstitution, the PKC alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This PKC alpha antibody is available for research use only.
Storage Conditions
After reconstitution, the PKC alpha antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
IHC staining of FFPE human breast cancer tissue with PKC alpha antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

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