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PRKCA Antibody / PKC alpha

CAT: 0800-V8687-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8687-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P17252
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
133
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the PRKCA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PRKCA antibody is available for research use only.
Storage Conditions
Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PRKCA antibody should be determined by the researcher.
Location
Cytoplasm, cell membrane, nucleus
Image Legend
IHC staining of FFPE human tonsil with PRKCA antibody (clone 133) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P17252

Protein kinase C alpha type

KPCA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17252
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRKCA
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PKC-A, PKC-alpha
EC number
2.7.11.13
Processing
—
Secondary accessions
B5BU22, Q15137, Q32M72, Q96RE4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Biological process

AngiogenesisApoptosisCell adhesion

Ligand

ATP-bindingCalciumMetal-bindingNucleotide-bindingZinc

Cellular component

Cell membraneCytoplasmMembraneMitochondrionNucleus

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

Disease

Proto-oncogene

Domain

RepeatZinc-finger
Product Datasheet
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