PRKCA Antibody / PKC alpha

• Description:

  Protein Kinase C alpha (PKC) is involved in the regulation of cell proliferation during cell cycle progression. This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Clone 133 recognizes the isoform of PKC and binds to a sequence at the C terminus of PKC to detect its expression in vitro.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P17252

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Full-length protein was used as the immunogen for the PRKCA antibody. The epitope has been mapped to amino acids PQFVHPILQSAV at the C terminus.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  133

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the PRKCA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PRKCA antibody is available for research use only.

• Storage Conditions:

  Store the PRKCA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PRKCA antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, cell membrane, nucleus

• Image Legend:

  IHC staining of FFPE human tonsil with PRKCA antibody (clone 133) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.