Actin Beta Antibody / ACTB

• Description:

  All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. a-Actin expression is limited to various types of muscle, whereas -Actin and g-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P60709

• Host:

  Mouse

• Reactivity:

  Human, Mouse and Rat

• Immunogen:

  Recombinant human protein was used as the immunogen for the Actin Beta antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ACTB/1109

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Aliquot the Actin Beta antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Actin Beta antibody is available for research use only.

• Storage Conditions:

  Aliquot the Actin Beta antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Actin Beta antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm

• Image Legend:

  IHC staining of FFPE human tonsil tissue with Actin Beta antibody (clone ACTB/1109) . Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.