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Beta Actin Antibody / ACTB

CAT: 0800-V5200-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V5200-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha-Actin expression is limited to various types of muscle, whereas Beta-Actin and Gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P60709
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the Beta Actin antibody.
Clonality
Monoclonal
Isotype
IgG
Clone
ACTB/2370
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Beta Actin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Beta Actin antibody is available for research use only.
Storage Conditions
Aliquot the Beta Actin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Beta Actin antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human spleen tissue with Beta Actin antibody (ACTB/2370) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P60709

Actin, cytoplasmic 1

ACTB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P60709
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ACTB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.4.-
Processing
—
Secondary accessions
P02570, P70514, P99021, Q11211, Q64316, Q75MN2, Q96B34, Q96HG5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondMethylationOxidationUbl conjugation

Ligand

ATP-bindingNucleotide-binding

Cellular component

CytoplasmCytoskeletonNucleus

Disease

DeafnessDisease variantDystoniaIntellectual disability

Molecular function

Hydrolase

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