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Recombinant ITGA4 Antibody

CAT: 0800-R20285-0.1MLSize: 100 µLDry Ice: NoHazardous: No
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CAT#:0800-R20285-0.1MLSize:100 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This recombinant ITGA4 antibody reacts to the cytoplasmic domain of human Integrin alpha 4.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1:1000-1:5000 (1), Western blot: 1:1000-1:2000
UniProt
P13612
Host
Rabbit
Reactivity
Human
Immunogen
A peptide corresponding to the C-terminus of human Integrin alpha 4 was used as the immunogen for this recombinant ITGA4 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
RM268
Type
Recombinant
Applications
WB, IHC
Purity
Protein A purified from animal origin-free supernatant
Format
Purified
Buffer
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Reconstitution
Store the recombinant ITGA4 antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Limitations
This recombinant ITGA4 antibody is available for research use only.
Storage Conditions
Store the recombinant ITGA4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol) .
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the recombinant ITGA4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Location
Cytoplasmic, membranous
Image Legend
Western blot testing of human Jurkat cell lysate with recombinant ITGA4 antibody at 1:1000. Predicted molecular weight ~115 kDa, routinely observed at ~150 kDa.

UniProtKB · P13612

Integrin alpha-4

ITA4_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13612
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ITGA4
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DPG4, Q7Z4L6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Domain

RepeatSignalTransmembraneTransmembrane helix
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