Products for Research Use Only

Itga4 Antibody / Integrin alpha 4

CAT: 0800-FY12338Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-FY12338Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Specifications
Western Blot: 0.25-0.5 µg/mL, Flow cytometry: 1-3ug/million cells, ELISA: 0.1-0.5 µg/mL
UniProt
Q00651
Host
Rabbit
Reactivity
Mouse, Rat
Immunogen
E.coli-derived mouse Integrin Alpha 4/Itga4 recombinant protein (Position: Y41-D963) was used as the immunogen for the Itga4 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, FACS, ELISA
Purity
Immunogen affinity purified
Format
Lyophilized
Buffer
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Limitations
This Itga4 antibody is available for research use only.
Storage Conditions
After reconstitution, the Itga4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml
Applications Notes
Optimal dilution of the Itga4 antibody should be determined by the researcher.
Image Legend
Flow Cytometry analysis of NIH/3T3 cells using anti-Integrin Itga4 antibody. Overlay histogram showing NIH/3T3 cells stained with (Blue line) . The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Integrin Itga4 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

UniProtKB · Q00651

Integrin alpha-4

ITA4_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q00651
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Itga4
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumMetal-binding

Biological process

Cell adhesion

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

IntegrinReceptor

Cellular component

Membrane

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix