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SIRT1 Antibody

CAT: 0800-F52857-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F52857-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SIRT1 is a NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. [UniProt]
CAS Number
9007-83-4
Specifications
IHC (Paraffin) : 1:25, Immunofluorescence: 1:25, Western blot: 1:1000
UniProt
Q923E4
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 566-601 of the mouse protein was used as the immunogen for the SIRT1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IHC, IF, WB, ELISA
Purity
Antigen affinity
Format
Antigen affinity purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Store at 4oC for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SIRT1 antibody is available for research use only.
Storage Conditions
Store at 4°C for up to one month. For long term, aliquot the SIRT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting points. Titration of the SIRT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from mouse F9 cell line using SIRT1 antibody at 1:1000. Predicted molecular weight ~80kDa but is routinely observed at 110~120kDa due to post-translational modification.

UniProtKB · Q923E4

NAD-dependent protein deacetylase sirtuin-1

SIR1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q923E4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sirt1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
2.3.1.286
Processing
—
Secondary accessions
Q9QXG8
Protein keywords

PTM

AcetylationMethylationPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisBiological rhythmsDifferentiationMyogenesisTranscriptionTranscription regulation

Cellular component

CytoplasmMitochondrionNucleus

Molecular function

Developmental proteinTransferase

Ligand

Metal-bindingNADZinc

Technical term

Reference proteome

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