PAK3 Antibody

• Description:

  PAK3, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. PAK3 interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1. It shows highly specific binding to the SH3 domains of phospholipase C-gamma and of adapter protein NCK. This protein is highly expressed in postmitotic neurons of the developing and postnatal cerebral cortex and hippocampus. PAK3 is autophosphorylated when activated by CDC42/p21. Defects in PAK3 are the cause of non-specific X-linked nonsyndromic mental retardation type 30 (MRX30) . The protein structure contains 1 CRIB domain.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100

• UniProt:

  O75914

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 218-247 from the human protein was used as the immunogen for this PAK3 antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Reconstitution:

  Aliquot the PAK3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This PAK3 antibody is available for research use only.

• Storage Conditions:

  Aliquot the PAK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the PAK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC analysis of FFPE human breast carcinoma tissue stained with the PAK3 antibody