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Anti-GAPDH Antibody

CAT: 0800-F50849-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
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CAT#:0800-F50849-0.4MLSize:0.4 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1000, IHC (Paraffin) : 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
P04406
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 62-91 from the human protein was used as the immunogen for this anti-GAPDH antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
IF, WB, IHC, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the anti-GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This anti-GAPDH antibody is available for research use only.
Storage Conditions
Aliquot the anti-GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the anti-GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot analysis of lysate from A375 cell line using anti-GAPDH antibody diluted at 1:500. Predicted molecular weight ~36kDa.

UniProtKB · P04406

Glyceraldehyde-3-phosphate dehydrogenase

G3P_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04406
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GAPDH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GAPDH
EC number
1.2.1.12
Processing
—
Secondary accessions
E7EUT4, P00354, Q53X65
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationADP-ribosylationGlycoproteinIsopeptide bondMethylationOxidationPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisGlycolysisImmunityInnate immunityTranslation regulation

Cellular component

CytoplasmCytoskeletonMembraneNucleus

Ligand

NAD

Molecular function

OxidoreductaseTransferase

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