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GAPDH Antibody

CAT: 0800-F50850-0.4MLSize: 0.4 mLDry Ice: NoHazardous: No
CAT#:0800-F50850-0.4MLSize:0.4 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.
CAS Number
9007-83-4
Specifications
Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50
UniProt
P04406
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 233-259 from the human protein was used as the immunogen for this GAPDH antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, IHC, FACS, IF, ELISA
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GAPDH antibody is available for research use only.
Storage Conditions
Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Prediction Reactivity
Chicken, Mouse, Pig, Rat
Image Legend
Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using GAPDH antibody; Ab was diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.

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