GAPDH Antibody

• Description:

  The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.

• Specifications:

  Western blot: 1:1000-8000, Immunofluorescence: 1:25, IHC (Paraffin) : 1:25

• UniProt:

  P04406

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant protein was used to produce this monoclonal GAPDH antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  1A10A10

• Applications:

  WB, IF, IHC, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This GAPDH antibody is available for research use only.

• Storage Conditions:

  Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• CAS Number:

  9007-83-4

• Image Legend:

  GAPDH antibody WB at 1:1000 dilution. Lane 1: A431 lysate; 2: rat C6; 3: HeLa; 4: HUVEC; Predicted band size : 36 kDa.