GAPDH Antibody

• Description:

  GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.

• Specifications:

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt:

  P04406

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 233-259 from the human protein was used as the immunogen for this GAPDH antibody.

• Clonality:

  Polyclonal

• Isotype:

  Ig

• Applications:

  WB, IHC, FACS, IF, ELISA

• Purity:

  Purified

• Format:

  Purified

• Buffer:

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution:

  Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This GAPDH antibody is available for research use only.

• Storage Conditions:

  Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes:

  Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity:

  Chicken, Mouse, Pig, Rat

• CAS Number:

  9007-83-4

• Image Legend:

  Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using GAPDH antibody; Ab was diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.