GAPDH Antibody

• Description :

  GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD) . The enzyme exists as a tetramer of identical chains.

• Specifications :

  Western blot: 1:1000, IHC (Paraffin) : 1:50-1:100, Flow Cytometry: 1:10-1:50, Immunofluorescence: 1:10-1:50

• UniProt :

  P04406

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 233-259 from the human protein was used as the immunogen for this GAPDH antibody.

• Clonality :

  Polyclonal

• Isotype :

  Ig

• Applications :

  WB, IHC, FACS, IF, ELISA

• Purity :

  Purified

• Format :

  Purified

• Buffer :

  In 1X PBS pH 7.4 with 0.09% sodium azide

• Reconstitution :

  Aliquot the GAPDH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This GAPDH antibody is available for research use only.

• Storage Conditions :

  Aliquot the GAPDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  In 1X PBS, pH 7.4, with 0.09% sodium azide

• Applications Notes :

  Titration of the GAPDH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

• Prediction Reactivity :

  Chicken, Mouse, Pig, Rat

• CAS Number :

  9007-83-4

• Image Legend :

  Western blot analysis of lysate from HeLa, HUVEC cell line (left to right) using GAPDH antibody; Ab was diluted at 1:1000 for each lane. Predicted molecular weight ~36kDa.