TIGIT Detection Set

• Background:

  TIGIT Antibody: The T cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins. It is expressed on several classes of T cells including follicular B helper T cells (TFH) . TIGIT has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses (1) . Similar to other immune checkpoint proteins such as PD-1, TIGIT is upregulated on exhausted T cells in chronic viral infections and cancer. Blockade of both TIGIT and PD-1 pathways leads to tumor rejection in mice suggesting that it may be of therapeutic use against cancer (2) .

• Host:

  Llama

• Reactivity:

  Human

• Immunogen:

  TIGIT antibodies were raised against a recombinant protein corresponding to amino acids 22-141 of human TIGIT.

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Detection Sets

• Purification:

  TIGIT Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein. They also contains a myc-tag for detection.

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  TIGIT Antibodies are supplied in PBS.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  TIGIT antibodies should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  Sdab

• Background Reference 01:

  Stanietsky N, Simic H, Arapovic J, et al. The interaction of TIGIT with PVR and PVRL2 inhibits human NK cell cytotoxicity. Proc Natl Acad Sci USA 2009; 106:17858-63.

• Background Reference 02:

  Johnston RJ, Comps-Agrar L, Hackney J, et al. The immunoreceptor TIGIT regulates antitumor and antiviral CD8 (+) T cell effector function. Cancer Cell 2014; 26:923-37.

• Tested Applications:

  ELISA, IHC-P, ICC, IF, Flow

• Physical Properties:

  Liquid