Junctophilin Detection Set

• Background:

  For images please see PDF data sheet

• Reactivity:

  Human

• Immunogen:

  Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

• Clonality:

  Polyclonal

• Type:

  Detection Sets

• Purification:

  Antibodies are supplied as affinity chromatography purified IgG.

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  PBS containing 0.02% sodium azide.

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Stable at 4˚ C for three months, store at -20˚ C for up to one year.

• Applications Notes:

  These polyclonal antibodies can be used for detection of JPH1 - 4 by immunoblot at 1 - 2 μ g/mL, and for detection of JPH1 - 4 by immunohistochemistry at 2.5 - 5 μ g/mL, and Immunoflourescence.

• Background Reference 01:

  Takeshima H, Komazaki S, Nishi M, et al. Junctophilins: a novel family of junctional membrane complex proteins. Mol. Cell. 2000; 6:11-22.

• Background Reference 02:

  Kakizawa S, Kishimoto Y, Hashimoto K, et al. Junctophilin-mediated channel crosstalk essential for cerebellar synaptic plasticity. EMBO J. 2007; 26:1924-33.

• Background Reference 03:

  Nishi M, Sakagami H, Komazaki S, et al. Coexpression of junctophilin type 3 and type 4 in brain. Brain Res. Mol. Brain Res. 2003; 118:102-10.

• Background Reference 04:

  Phimister AJ, Lango J, Lee EH, et al. Conformation-dependent stability of Junctophilin 1 (JP1) and Ryanodine Receptor type 1 (RyR1) channel complex is mediated by their hyper-reactive thiols. J. Biol. Chem. 2007; 282:8867-77.

• Tested Applications:

  WB, IHC, IF

• Physical Properties:

  Liquid