LAG3 Detection Set

• Background:

  The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2) . It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4) . LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.

• Host:

  Llama

• Reactivity:

  Human

• Immunogen:

  LAG3 antibodies were raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Detection Sets

• Purification:

  LAG3 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein. They also contains a myc-tag for detection.

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  LAG3 Antibodies are supplied in PBS.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  LAG3 antibodies should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  Sdab

• Background Reference 01:

  Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:3216–21.

• Background Reference 02:

  Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:619–22.

• Background Reference 03:

  Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:1885–91.

• Background Reference 04:

  Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:970–9.

• Tested Applications:

  ELISA, IHC-P, IF, Flow

• Physical Properties:

  Liquid