PD1 Detection Set

• Background:

  PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC) . T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

• Host:

  Llama

• Reactivity:

  Human

• Immunogen:

  PD-1 antibodies were raised against a recombinant protein corresponding to amino acids 25 - 167 of human PD-1.

• Clonality:

  Monoclonal

• Conjugation:

  Unconjugated

• Type:

  Detection Sets

• Purification:

  PD-1 Antibodies are affinity chromatography purified via Nickel column. Antibodies are supplied as a His-tagged purified protein. They also contains a myc-tag for detection.

• Concentration:

  Antibody 1 mg/mL

• Buffer:

  PD-1 Antibodies are supplied in PBS.

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  PD-1 antibodies should be stored in working aliquots at -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  Sdab

• Background Reference 01:

  Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.

• Background Reference 02:

  Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.

• Background Reference 03:

  Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.

• Background Reference 04:

  Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.

• Tested Applications:

  ELISA

• Physical Properties:

  Liquid