LGR5 / GPR49 Recombinant Protein

• Background:

  LGR5 (also known as GPR49) is a seven-transmembrane protein of the class A Rhodopsin-like family of GPCRs. LGR5 and LGR4 bind the R-spondins with high affinity and mediate the potentiation of Wnt/β-catenin signaling by enhancing Wnt-induced LRP6 phosphorylation. The LGR5/RSPO complex could promote Wnt signalling via the neutralization of two transmembrane E3 ligases, RNF43 and ZNRF3.

• NCBI Gene ID:

  8549

• Swiss Prot:

  O75473

• Accession Number:

  NP_003658.1

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  62.0 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.0 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  LeucinerichrepeatcontainingGprotein-coupledreceptor5

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Morgan RG. et al. Br J Cancer. 2018 May 29; 118 (11) : 1410–1418.

• Background Reference 02:

  Yue Z. et al. FASEB J. 2018 May;32 (5) :2422-2437.

• Background Reference 03:

  De Lau W. et al. Genes & development. 2014;28:305–16.

• Other Product Names:

  LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3