B7-2 Recombinant Protein

• Background:

  Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ. CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation) . CD86 works in tandem with CD80 to prime T cells. Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival. Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-kappaB-signaling pathway in macrophages. CD86 thus is regarded as a promising candidate for immune therapy. CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.

• NCBI Gene ID:

  12524

• Swiss Prot:

  P42082-1

• Accession Number:

  NP_062261.3

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Immunology

• Buffer:

  Tris with Glycine, Arginine and NaCl, pH7.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  52 kDa

• Applications Notes:

  This recombinant protein can be used for E, WB. For research use only.

• NCBI Official Name:

  T-lymphocyte activation antigen CD86 precursor

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Chen C, et al., 1994, J. Immunol. 152 (10) : 4929–36.

• Background Reference 02:

  Yadav, D. et al., 2007, J. Immunol. 178: 6236-6241.

• Background Reference 03:

  Steidl C, et al., 2010, N. Engl. J. Med. 362 (10) : 875–85.

• Other Product Names:

  CD86, B7-2, B70, CD28LG2, LAB72, MGC34413

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3