B7-H3 (4Ig) / B7-H3b Recombinant Protein

• Background:

  Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern.

• NCBI Gene ID:

  80381

• Swiss Prot:

  Q5ZPR3

• Accession Number:

  NP_001019907.1

• Tag:

  Fc Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Purity:

  >95% as determined by reduced SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  73.1 kDa

• Applications Notes:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 73.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  CD276 molecule

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Chapoval AI., et al. 2001, Nat Immunol., 2 (3), 269-74.

• Background Reference 02:

  Steinberger P., et al. 2004, J Immunol., 172 (4), 2352-9.

• Background Reference 03:

  Sun M., et al. 2002, J Immunol., 168 (12), 6294-7.

• Other Product Names:

  4Ig-B7-H3, B7-H3, CD276, PSEC0249, UNQ309/PRO352, B7 homolog 3

• Tested Applications:

  ELISA, WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3