CD45 / PTPRC Recombinant Protein

• Background:

  CD45 is a receptor protein tyrosine phosphatase, also known as Ly-5 or leukocyte common antigen. CD45 mainly involves in the initiation of T cell receptor signaling by controlling the activation of the Src family protein-tyrosine kinases Lck and Fyn. CD45 deficiency causes in T- and B-lymphocyte dysfunction in the form of severe combined immune deficiency. It also takes a significant role in autoimmune diseases and cancer as well as in infectious diseases including fungal infections.

• NCBI Gene ID:

  5788

• Swiss Prot:

  P08575

• Accession Number:

  NP_002829.3

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  HEK293 cells

• Field of Research:

  Infectious Disease, Neuroscience

• Purity:

  >90% as determined by SDS-PAGE.

• Buffer:

  PBS, pH7.4

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  62.8 kDa

• Applications Notes:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62.8 kDa. The protein migrates as 120-170 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• NCBI Official Name:

  ProteintyrosinephosphatasereceptortypeC

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shen F.W. et al. Immunogenetics 24 (1986) 146–149.

• Background Reference 02:

  Charbonneau H. et al. Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 7182–7186.

• Background Reference 03:

  Rheinländer A. et al. Immunol Lett. 2018 Apr; 196:22-32.

• Other Product Names:

  CD45, PTPRC, L-CA, T200

• Tested Applications:

  WB

• Species:

  Human

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3