Recombinant Mouse CD45/PTPRC Protein

• Background:

  The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Protein tyrosine phosphatase, receptor type C (CD45), also known as PTPRC is a member of the protein tyrosine phosphatase (PTP) family which is known for its function to serve as signaling molecules and to regulate a variety of cellular processes such as cell proliferation, differentiation, mitotic cycle and oncogenic transformation. CD45 is found expression specifically in hemotopietic cells. CD45 consists of an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It serves as an essential regulator of T-cell and B-cell antigen receptor signaling through either direct interaction with components of the antigen receptor complexs or by activating various Src family kinases required for the antigen receptor signaling and it also can suppress JAK kinases.

• Synonyms:

  B220; CD45R; Cd45; L-CA; Ly-5; Lyt-4; T200; loc

• UniProt:

  P06800

• Accession Number:

  AAA39458.1

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  None

• Sequence:

  Arg453-Ser1152

• Applications:

  ELISA; Enzyme

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  1. The specific activity was determined to be 9168 nmol/min/mg using p-nitrophenyl phosphate as substrate. 2. Immobilized mouse PTPRC (453-1152) at 10 μg/ml (100 μl/well) can bind biotinylated human Galectin-1 with a linear range of 0.31-2. 5 μg/ml.

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  81.0 kDa

• Observed Molecular Weight:

  93 kDa

• Formulation:

  Supplied as sterile solution of 20mM Tris, 500mM NaCl, 10% glycerol, 3mM DTT, pH 7.4

• Species:

  Mouse