ATG7 Recombinant Protein

• Background:

  Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1 (3) . Another member of the autophagy family of proteins is APG7 which was identified in yeast as a ubiquitin-E1-like enzyme; this function is conserved in the mammalian homolog (4) . In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (5) . It has been suggested that caspase-8 may alter APG7 levels and thus the APG7 program of autophagic cell death (6) .

• NCBI Gene ID:

  74244

• Swiss Prot:

  Q9D906

• Accession Number:

  AAH58597

• Tag:

  Fusion Partner:

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Field of Research:

  Autophagy

• Purity:

  ~95%

• Buffer:

  1X PBS containing 10% glycerol

• Shipping Conditions:

  Dry Ice

• Storage Conditions:

  Store in working aliquots at -70˚ C. Avoid freeze/thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

• Calculated Molecular Weight:

  30 kDa (Calculated)

• Applications Notes:

  This recombinant protein can be used for WB and ELISA. For research use only.

• NCBI Official Name:

  Autophagy related 7

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene . 2004; 23:2891-906.

• Background Reference 02:

  Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.

• Background Reference 03:

  Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.

• Background Reference 04:

  Mizushima N, Noda T, Yoshimori T, et al. A protein conjugation system essential for autophagy. Nature 1998; 395:395-8.

• Other Product Names:

  ATG7 Recombinant Protein: Agp7, Apg7l, Atg7l, Gm21553, 1810013K23Rik

• Tested Applications:

  ELISA, WB

• Species:

  Mouse

• Protein ID:

  37589293

• Physical Properties:

  Liquid

• CAS Number:

  9000-83-3