Protein G Recombinant Protein

• Background:

  Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.

• Swiss Prot:

  P19909

• Tag:

  His Tag

• Type:

  Recombinant Proteins

• Source:

  E. coli

• Purity:

  >98% as determined by SDS-PAGE.

• Buffer:

  0.1 M NaH2PO4, pH8.5

• Shipping Conditions:

  Room Temp

• Storage Conditions:

  Lyophilized Protein should be stored at -20˚ C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚ C or -70˚ C. Avoid repeated freeze-thaw cycles.

• Applications Notes:

  This recombinant protein can be used for WB. For research use only.

• Background Reference 01:

  Sjobring U, Bjorck L, Kastern W, et al., 1991, J Biol Chem 266 : 399–405.

• Other Product Names:

  RPG, Protein G

• Tested Applications:

  WB

• Species:

  Others

• Physical Properties:

  Lyophilized

• CAS Number:

  9000-83-3