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DsRed Antibody (Biotin)

CAT: 0013-GTR18184598Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184598Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RFP antibody (Biotin)
Product Name Alternative
Rabbit anti-RFP antibody biotin conjugation, biotin conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
UniProt
Q9U6Y8
Reactivity
Other
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Target
DsRed
Clonality
Polyclonal
Conjugation
Biotin
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000 - 1:80,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Purity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, and purified and partially purified Red Fluorescent Protein (Discosoma) . No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.
Form
Lyophilized
Storage Conditions
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Polyclonal anti-RFP is designed to detect RFP and its variants. This rabbit anti-RFP Antibody Biotin Conjugated antibody has been tested by ELISA and western blot. This product can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP . Optimal titers for applications should be determined by the researcher.
Tested Applications
ELISA, IHC, WB
Host or Source
Rabbit
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgG

UniProtKB · Q9U6Y8

Red fluorescent protein drFP583

RFP_DISSP · Discosoma sp.

View on UniProt ↗
Primary accession
Q9U6Y8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
—
Protein existence
1: Evidence at protein level
Organism
Discosoma sp. (Sea anemone)
Taxonomy ID
86600
Alternative names
DsRed
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structure

Ligand

Chromophore

Biological process

Luminescence

Molecular function

Photoprotein

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