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DsRed Antibody

CAT: 0013-GTR18207368Size: 50 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18207368Size:50 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recombinant RFP antibody
Product Name Alternative
Recombinant anti-RFP antibody, Red Fluorescent Protein antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
UniProt
Q9U6Y8
Reactivity
Other
Target
DsRed
Clonality
Recombinant
Clone
RFP40
Conjugation
Unconjugated
Source
E. Coli
Concentration
1.0 mg/mL
Dilution
ELISA: 1:31,000, WB: 1:1000
Purity
Anti-RFP (VHH) Antibody is a recombinant antibody. The clone was isolated from a library prepared from a hyper-immunized llama host and purified by affinity chromatography from bacterial culture. Expect reactivity against RFP-labeled probes and proteins.
Form
Liquid (sterile filtered)
Storage Conditions
Store at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-RFP is a his-tagged monoclonal recombinant antibody designed to detect Red Fluorescent Protein and its conjugates. This antibody has been tested by dot blot and ELISA and is intended for use in immunological assays including ELISA, immunofluorescence and fluorescence activated cell sorting (FACS) . The antibody can be labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection of RFP-conjugated molecules by immunofluorescence. Secondary detection can be achieved using conjugated anti-His tag or anti-VHH antibodies. Optimal titers for applications should be confirmed by the end user. This antibody is not suitable for Western blot detection of denatured RFP.
Tested Applications
DOT, ELISA, WB
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 20% (v/v) Glycerol; Buffer: 1X PBS, pH 7.4

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