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DsRed Antibody

CAT: 0013-GTR18184621Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18184621Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RFP antibody
Product Name Alternative
Chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
UniProt
Q9U6Y8
Reactivity
Other
Immunogen
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Target
DsRed
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:1,000 - 1:3,000
Purity
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by Western blot.
Form
Liquid (sterile filtered)
References & Citations
Kivelä, Riikka et al. The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation Nat Commun, 7, 13124 (2016)
Pubmed id
27731315
Storage Conditions
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Notes
For research use only.
Applications Notes
Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Tested Applications
ELISA, WB
Host or Source
Gallus
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgY

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