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DsRed Antibody

CAT: 0013-GTR18184622Size: 25 µLDry Ice: NoHazardous: No
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CAT#:0013-GTR18184622Size:25 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
RFP antibody
Product Name Alternative
Chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
UniProt
Q9U6Y8
Reactivity
Other
Immunogen
Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Target
DsRed
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
1.0 mg/mL
Dilution
ELISA: 1:10,000, WB: 1:2,000 - 1:3,000
Purity
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot.
Form
Liquid (sterile filtered)
Storage Conditions
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL) . To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Notes
For research use only.
Applications Notes
Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA, SDS-PAGE, and western blot to detect RFP. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
Tested Applications
ELISA, WB
Host or Source
Gallus
Preservative
Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Isotype
IgY

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