Anti-HLA Class I PE

• Description:

  HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

• Certification:

  RUO

• Immunogen:

  COS-7 African green monkey kidney cells

• Target Antigen:

  HLA Class I

• Clone:

  MEM-123

• Applications:

  FC (QC tested)

• Concentration:

  0.1 mg/mL

• Format:

  PE

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.

• Applications Notes:

  Flow cytometry: Recommended dilution: 2-4 μg/ml.

• Species:

  Cow, Human, Non-human primates

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG3