Anti-HLA Class I Purified

Anti-HLA Class I Purified

• Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
• Specifications: The antibody MEM-123 reacts with an extracellular epitope of all human classical MHC Class I molecules (major histocompatibility complex) in native cell-surface forms as well as with human HLA-G cDNA transfected cells. MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. The antibody MEM-123 completely blocks binding of classical W6/32 to surface-expressed HLA-G, but does not cross-blocks the antibody MEM-G/9.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Human, Non-human primates
• Immunogen: COS-7 African green monkey kidney cells
• Target Antigen: HLA Class I
• Isotype: IgG3
• Clone: MEM-123
• Applications: Flow cytometry: Recommended dilution: 4 μg/mL.
• Validated Applications: FC, IP, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Storage Conditions: Store at 2-8°C. Do not freeze.