Anti-HLA Class I Purified

• Description:

  HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.

• Certification:

  RUO

• Reactivity:

  Human

• Immunogen:

  Leukocytes of a patient suffering with LGL type leukemia.

• Target Antigen:

  HLA Class I

• Clone:

  MEM-81

• Applications:

  FC (QC tested)

• Concentration:

  1 mg/mL

• Format:

  Purified

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Valenzuela NM, Mulder A, Reed EF: HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging Fcγ Rs. J Immunol. 2013 Jun 15;190 (12) :6635-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23690477

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody MEM-81 reacts with an extracellular epitope on human classical MHC Class I molecules in native cell-surface forms. MHC Class I molecules (MHC Class Ia) are expressed on the surface of human nucleated cell types.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Isotype:

  Mouse IgG1