Anti-HLA Class I Purified

Anti-HLA Class I Purified

• Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
• Specifications: The antibody MEM-81 reacts with an extracellular epitope on human classical MHC Class I molecules in native cell-surface forms. MHC Class I molecules (MHC Class Ia) are expressed on the surface of human nucleated cell types.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Leukocytes of a patient suffering with LGL type leukemia.
• Target Antigen: HLA Class I
• Isotype: IgG1
• Clone: MEM-81
• Applications: Flow cytometry: Recommended dilution: 1-5 μg/mL.
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Valenzuela NM, Mulder A, Reed EF: HLA class I antibodies trigger increased adherence of monocytes to endothelial cells by eliciting an increase in endothelial P-selectin and, depending on subclass, by engaging Fc947Rs. J Immunol. 2013 Jun 15190(12):6635-50. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23690477,
• Storage Conditions: Store at 2-8°C. Do not freeze.