Anti-HLA Class I FITC

Anti-HLA Class I FITC

• Background: HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
• Specifications: The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Cow, Non-human primates, Human, Cat
• Negative Species: Rabbit
• Immunogen: Membrane of human tonsil cells
• Target Antigen: HLA Class I
• Isotype: IgG2a
• Clone: W6/32
• Applications: Flow cytometry: Recommended dilution: 1-3 μg/mL.
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: FITC
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct20(2):115-26.
, URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=3316135&query_hl=24&itool=pubmed_docsum ,*Ladasky JJ, Shum BP, Canavez F, Seuanez HN, Parham P: Residue 3 of beta2-microglobulin affects binding of class I MHC molecules by the W6/32 antibody. Immunogenetics. 1999 Apr49(4):312-20. , URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=10079295&query_hl=8&itool=pubmed_docsum ,*Le Discorde M, Moreau P, Sabatier P, Legeais JM, Carosella ED: Expression of HLA-G in human cornea, an immune-privileged tissue. Hum Immunol. 2003 Nov64(11):1039-44., URL: https://www.ncbi.nlm.nih.gov/pubmed/14602233?ordin,*Tran TM, Ivanyi P, Hilgert I, Brdicka T, Pla M, Breur B, Flieger M, Ivaskova E, Horejsi V: The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics. 2001 Aug53(6):440-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/11685454?dopt=,*Shields MJ, Ribaudo RK: Mapping of the monoclonal antibody W6/32: sensitivity to the amino terminus of beta2-microglobulin. Tissue Antigens 1998 May51(5):567-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/9672158?dopt=A,*Jacobsen, C. N. et al. (1993): Reactivities of 20 anti-human monclonal antibodies with leucocytes from ten different animal species. Vet. Immunopathol. 39: 461 - 466., URL: https://www.ncbi.nlm.nih.gov/pubmed/8116221?dopt=A,*Kievits F, Ivanyi P: Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin.Hum Immunol. 1987 Oct20(2):115-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/3316135?dopt=A,*Stern, P. et al. (1987): Class I-like MHC molecules expressed by baboon placental synctiotrophoblast. Journal of Immunology. 138 (4): 1088 - 1091., URL: https://www.ncbi.nlm.nih.gov/pubmed/3543121?dopt=A,*Neefjes, J.J. et al. (1986): A biochemical characterization of feline MHC products: unusually high expression of class II antigens on peripheral blood lymphocytes. Immunogenetics 23: 341-347., URL: https://www.ncbi.nlm.nih.gov/pubmed/3458670?dopt=A,*Brodsky, F.M. et al. (1982): Evolution of HLA antigenic determinants: species cross reactions of monoclonal antibodies. Immunogenetics 15: 151-166., URL: https://www.ncbi.nlm.nih.gov/pubmed/6174442?dopt=A,*Barnstable, C. J., et al. (1978) Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens - new tools for genetic analysis. Cell. 14: 9 - 20., URL: https://www.ncbi.nlm.nih.gov/pubmed/667938?dopt=Ab,*Polakova K, Karpatova M, Russ G: Dissociation of beta 2-microglobulin is responsible for selective reduction of HLA class I antigenicity following acid treatment of cells. Mol Immunol. 1993 Oct30(14):1223-30., URL: https://pubmed.ncbi.nlm.nih.gov/7692238/,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.